ANTI FGF2 BFGF; CL BFM2 500UG

Code: 05-118 D2-231

Analysis Note

ControlFetal liver or kidney tissue.

Application

Research CategorySignaling

Detect FGF-2/basic FGF using this Anti-FGF-2/basi...


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Analysis Note

ControlFetal liver or kidney tissue.

Application

Research CategorySignaling

Detect FGF-2/basic FGF using this Anti-FGF-2/basic FGF Antibody, clone bFM-2 validated for use in IH, RIA & WB.

Research Sub CategoryGrowth Factors & Receptors

Immunohistochemistry:
Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 µg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Upstate recommends anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) for neutralizing activity.

Immunogen

Purified bovine brain basic FGF (FGF-2, HBGF-2). Clone bFM-2.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.4, purified by ammonium sulfate precipitation and DEAE Sephacryl chromatography. Frozen solution.

Format: Purified

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Quality

Evaluated on basic FGF (Catalog #01-114).

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).

Specificity

This highly specific antibody does not cross-react with bovine acidic FGF (FGF-1, HBGF-1).

Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.

This antibody detects FGF-2/basic FGF.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

17.5 kDa

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clonebFM-2, monoclonal
Gene Informationhuman ... FGF2(2247)mouse ... Fgf2(281161), Fgf2(14173)rat ... Fgf2(54250)
isotypeIgG1κ
manufacturer/tradenameUpstate®
NCBI accession no.NM_002006.3
Quality Level100, 500
shipped indry ice
species reactivitybovine, rat, human, mouse
technique(s)western blot: suitable, radioimmunoassay: suitable, immunohistochemistry: suitable
UniProt accession no.P09038
This product has met the following criteria to qualify for the following awards:



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