Analysis Note

ControlFetal liver or kidney tissue.

Application

Research CategorySignaling

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 is an antibody against FGF-2/basic FGF (neutralizing) for use in NEUT, RIA.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Basic fibroblast growth factor, also known as bFGF or FGF2, is a member of the fibroblast growth factor family.
In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.
It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis.
Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined.

Immunogen

Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.

Epitope: a.a. 5-24

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.

Format: Purified

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Quality

Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.

Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.

Specificity

This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.

This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

17.5 kDa