Analysis Note

ControlHEK293 cell lysate

Application

Research CategoryCell Structure

This Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 is validated for use in WB, IP for the detection of Focal Adhesion Kinase.

Research Sub CategoryCytoskeletal Signaling

Immunohistochemistry Analysis: A previous lot detected Focal Adhesion Kinase at 1:100 dilution in mouse cortex tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src (Guan, J L and Shalloway, D). This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. Additional components of focal adhesions include actin, filamin, vinculin, talin, paxillin and tensin (Burridge, K, et al).

Immunogen

Epitope: Terminal

Recombinant fragment derived from the amino terminal region of chicken FAK, amino acid residues 103 to 553.

Linkage

Replaces: 04-591

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Focal Adhesion Kinase in 10 µg of HEK293 cell lysate.

Specificity

This antibody recognizes Focal Adhesion Kinase

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~125 kDa