Analysis Note

ControlNIH/3T3 cell lysate

Application

Research CategorySignaling

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH(P) (Venkateswaran, S., et al)

Detect Fibroblast Growth Factor Receptor 1 using this Anti-Fibroblast Growth Factor Receptor 1 Antibody, clone VBS1 validated for use in WB, IP, IH(P), ELISA.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The fibroblast growth factor receptors are members of the tyrosine kinase superfamily and consist of an extracellular ligand domain composed of three immunoglobulin-like domains, a single transmembrane helix domain, and an intracellular domain with tyrosine kinase activity. (Ornitz, DM. and Itoh, N) Monoclonal antibodies against the high affinity tyrosine kinase Fibroblast Growth Factor (FGF) receptor may help define receptor epitopes involved in FGF binding and signal transduction which mediate coronary and tumor angiogenesis. (Venkateswaran, S., et al).

Immunogen

FGF Receptor purified from bovine coronary venular endothelial cells.

Epitope: Extracellular

Linkage

Replaces: MAB125

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified mouse monoclonal IgM in 0.015 M potassium phosphate buffer containing 0.15 M NaCl, 0.1% sodium azide, pH 7.20.

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 0.78 µg/mL of this antibody detected Fibroblast Growth Factor Receptor 1 in 10 µg of NIH/3T3 cell lysate.

Specificity

This antibody recognizes the extracellular ligand-binding domain of Fibroblast Growth Factor Receptor 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~92 kDa