Analysis Note

ControlNIH/3T3 cell lysate

Application

Research CategorySignaling

Detect VEGF using this Anti-VEGF Antibody, clone VG1 validated for use in WB.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines.

Immunogen

Recombinant human VEGF 189aa isoform

Linkage

Replaces: MAB3734

Physical form

Unpurified mouse monoclonal IgG1κ in ascites fluid containing no preservatives.

Unpurified

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected VEGF on 10 µg of NIH/3T3 cell lysate.

Specificity

Detects the 121, 165, and 189 amino acid isoforms of VEGF.

Storage and Stability

Stable for 12 months at -20°C from date of receipt.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Approx. 12-28 kDa