Analysis Note

ControlSuggested Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is recommended as a positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at –20°C. The lysate is stable for 6 months at –20°C.

Application

Research CategorySignaling

Research Sub CategoryGeneral Post-translation Modification

Anti-Phosphoserine Antibody, clone 4A4 is an antibody against Phosphoserine for use in WB, IF, FC, IH(P), ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).

Immunogen

Phosphoserine coupled to KLH

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

50 µL of PBS with 0.05% sodium azide with 30% glycerol.

Protein G-Sepharose™ Chromatography

Format: Purified

Quality

Routinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.

Specificity

Serine-phosphorylated proteins from all species

Storage and Stability

2 years at -20°C from date of shipment.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.

Target description

Dependent upon the molecular weight of the serine phosphorylated protein being detected.