Analysis Note

ControlRat spinal cord tissue lysate

Application

Detect Desert hedgehog protein (DHH) using this Anti-Desert hedgehog protein (DHH) Antibody, clone 19D7.2 validated for use in WB, IH.

Immunohistochemistry Analysis: 1:600 dilution from a previous lot detected Desert hedgehog protein in malignant teratoma tissue.

General description

Desert Hedgehog (DHH) is one of three signaling molecules belonging to the hedgehog family, a group of intracellular signaling molecules characterized for their regulatory ability in developmental tissue patterning. DHH is an essential component to the integrity of peripheral nerve function and structure. There is also strong support that it is critical to normal, testicular development, and the survival of spermatocytes. DHH cleaves into two chains; the DHH N-product which is localized at the cell surface, and the DHH C-product which diffuses from the cell. Defects in expression are though to cause complete pure gonadal dysgenesis 46,XY type (GDXYM), and partial gonadal dysgenesis with minifascicular neuropathy 46, XY (PGD).

Immunogen

KLH-conjugated linear peptide corresponding to human Desert hedgehog protein.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blot in rat spinal cord tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Desert hedgehog protein on 10 µg of rat spinal cord tissue lysate.

Target description

~ 44 kDa observed