Analysis Note

ControlHL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ

Application

Detect phospho-Smad2 (Ser465/467) with Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S (Rabbit Monoclonal Antibody), that has been shown to work in WB.

General description

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 05-953

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Routinely evaluated by immunoblot analysis.

Specificity

Recognizes phosphorylated Smad2. No Cross-reactivity with Smad3.

Predicted to cross-react with X. laevis based on sequence homology.

Target description

55-60 kDa