Application

Western Blot: 1:1000-1:4000 dilution for the detection of phospho-KDR/Flk-1/VEGFR2 in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.

Research CategorySignaling

This Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W is validated for use in WB for the detection of phospho-KDR/Flk-1/VEGFR2 (Tyr1054).

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

KLH-conjugated, synthetic peptide encompassing the phosphorylated tyrosine residue 1054 of human KDR/Flk-1/VEGFR2.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Cultured supernantant with 0.05% sodium azide

Quality

Routinely evaluated by immunoblot in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.

Specificity

KDR/Flk-1/VEGFR2 phosphorylated on Tyrosine 1054.

Predicted to have broad species cross-reactivity based on sequence homology.

Storage and Stability

2 years at -20°C from date of shipment.

Target description

152 kDa