Analysis Note

ControlRecombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.

Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Anti-Histone H4 Antibody, pan is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex.

Immunoblot Analysis:
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).

Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex^® microspheres. The antibody recognized both modified and unmodified peptides.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

This antibody recognizes histone H4 independant of any post-translational modifications.

Immunogen

Synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28

Epitope: a.a. 25-28

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Luminex is a registered trademark of Luminex Corp

Linkage

Replaces: 05-858

Physical form

Cultured supernantant in 0.05% sodium azide.

Quality

Routinely evaluated by immunoblot using HeLa cell acid extract.

Specificity

Recognizes Histone H4.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

10 kDa