Analysis Note

ControlRIPA lysates from HEK293 cells.

Application

Research CategorySignalingApoptosis & Cancer

Detect TBK1 using this Anti-TBK1 Antibody, clone AOW9 validated for use in WB & IP.

Research Sub CategoryKinases & PhosphatasesImmunological Signaling

Immunoprecipitation:
1-4 µL of a previous lot immunoprecipitated TBK1 from 100 µg of HEK293 RIPA lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.

Immunogen

Peptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.

Epitope: a.a. 642-645

Linkage

Replaces: 05-856

Physical form

Cultured supernatant containing 0.05% sodium azide.

Quality

Evaluated by western blot on RIPA lysates from HEK293 cells.

Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.

Specificity

Predicted to cross-react with mouse, dog, and chicken based on sequence homology.

TBK1; Does not cross-react with IKKε, IKKα or IKKβ.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

85 kDa