Analysis Note

ControlRat brain

Application

Anti-phospho-GluR1 (Ser831) Antibody, clone N453 detects level of phospho-GluR1 (Ser831) & has been published & validated for use in WB.

Research CategoryNeuroscience

Western Blot Analysis:
A 1:1,000-1:4,000 dilution of this lot detected phosphorylated GluR1 from a rat brain microsomal preparation (Catalog # 12-144).

Research Sub CategoryNeurotransmitters & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

L glutamate is the major excitatory neurotransmitter in the central nervous system. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors that have been divided into 3 groups. Group I includes GRM1 and these receptors have been shown to activate phospholipase C. GRM1 expression has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries.

Immunogen

KLH-conjugated, synthetic peptide containing the sequence ...QQ[pS]INR30; in which pS corresponds to phospho-serine at position 831 of the GluR1 mature chain. Sequence is identical in human, rat, mouse and chicken.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 05-823

Physical form

Cultured supernantant in 0.05% sodium azide.

Quality

routinely evaluated by western blot

Specificity

Predicted cross-reactivity with human, mouse and chicken

Recognizes GluR1 phosphorylated on Serine 831.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Target description

106 kDa