Analysis Note

ControlHEK293 cell lysate.

Application

Research Sub CategoryPI3K, Akt, & mTOR Signaling

Research CategorySignaling

Anti-Akt1 Antibody, clone AW24 detects level of Akt1/PKBα & has been published & validated for use in WB & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Immunogen

Epitope: C-terminus

Sythetic peptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1/PKBα.

Linkage

Replaces: 05-796

Physical form

Cultured supernantant containing 0.05% sodium azide.

Format: Purified

Quality

Evaluated by western blot on RIPA lysates from human HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Akt1/PKBα in RIPA lysates from human HEK293 cells.

Specificity

This antibody recognizes Akt1/PKBα.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~60 kDa