Analysis Note

ControlSerum stimulated 3T3 cell lysate.

Application

Western Blot Analysis:
A 1:500-1:2,000 dilution of this lot detected phosphorylated ribosomal protein S6 in lysates from serum stimulated 3T3 cells.

Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27 is an antibody against phospho-Ribosomal Protein S6 (Ser235) for use in WB.

Research Sub CategoryPI3K, Akt, & mTOR Signaling

Research CategorySignalingApoptosis & Cancer

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that is known to be phosphorylated by both p70S6 Kinase as well as RSK as well as Ser240/244 which is thought to only be phosphorylated by p70S6K.

Immunogen

Synthetic peptide containing the sequence R30;RRL[pS]SLR30; in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus.

Epitope: Phosphorylated Ser235

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Cultured supernatant with 0.05% sodium azide.

Quality

Routinely evaluated by western blot on serum stimulated 3T3 cell lysate.

Specificity

Broad species cross-reactivity predicted, due to sequence homology.

Recognizes ribosomal protein S6 only when phosphorylated on Ser235.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~30 kDa