Analysis Note

ControlAcid extracted proteins from colcemid-arrested HeLa cells

Application

Research CategoryEpigenetics & Nuclear Function

Anti-phospho-CENP-A (Ser7) Antibody, clone NL41 is a high quality Rabbit Monoclonal Antibody for the detection of phospho-CENP-A (Ser7) & has been validated in Mplex, WB, ICC.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CENP-A is a variant version of histone H3 found only at centromeres. It is phosphorylated at serine 7 during mitosis.

Centromere protein A (CENP-A) is a 17 kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of α-satellite DNA into primary chromation structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.

Immunogen

peptide containing the sequence RRpSRK in which pS is phospho-serine corresponding to amino acid 7 of human CENP-A (centromere protein A)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 05-792

Physical form

Cultured supernantant in 0.05% sodium azide

Quality

routinely evaluated by immunoblot on acid extracted proteins from colcemid-arrested HeLa cells (Catalog #17-306)

Specificity

Others not tested.

CENP-A phosphorylated on serine 7

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~17kDa