Application

Immunoblot Analysis: 1:1,000 to 1:4,000 dilutions of this lot detected IRS1 in RIPA lysates from 3T3/A31 mouse fibroblasts.

Immunoprecipitation: 5-10µl of this lot immunoprecipitated IRS1 from 500µg of 3T3/A31 RIPA lysate.

Research CategorySignaling

Research Sub CategoryInsulin/Energy Signaling

Anti-IRS1 Antibody, clone AW58 detects level of IRS1 & has been published & validated for use in IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Insulin Receptor substrate proteins are the effectors of both Insulin and IGF-initiated signaling. They share PH and PTB domains near their n-termini, and multiple Tyr phosphorylation motifs in their c-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2.

Immunogen

Peptide corresponding to carboxy-terminal 14 amino acids (C-YASINFQKQPEDRQ) of rat liver IRS1

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 05-784

Physical form

Cultured supernantant in 0.05% sodium azide

Quality

routinely evaluated by immunoblot on in RIPA lysates from 3T3/A31 mouse fibroblasts

Specificity

IRS1

Storage and Stability

1 year at -20°C from date of shipment

Target description

Mr ~145-165kDa