Application

Immunoblotting (1:500 to 1:2000)

Optimal working dilutions must be determined by the end user

Research CategorySignaling

This Anti-PP2A Antibody, B subunit, B56 delta, clone H5D12 is validated for use in WB for the detection of PP2A.

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PP2A is a heterotrimeric phosphatase, ccomposed of A, B and C subunits. The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. Three families of B subunits have been identified thus far. The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B′ (or B56) family consists of at least seven splice variantsderived from five genes. RNA′s for the two members of the B′′ family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B′ subunits are conserved from yeast to mammals.

Immunogen

GST fusion protein corresponding to the N-terminus of human PP2A, B subunit, B56 delta

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1 antibody in PBS with 0.05% NaN3.

Format: Purified

Specificity

Human PP2A, B subunit, B56 delta. This monoclonal antibody is the delta isoform for the B56 family of protein phosphatase 2A.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.