Application

Anti-RhoG Antibody, clone 1F3 B3 E5 is an antibody against RhoG for use in WB.

General description

RhoG (ARGH) is a member of the Ras super-family of small G-proteins and a member of the Rac subfamily of Rho Family of GTPases. RhoG is a small G-protein (GTPase) who is activated by a GEF (guanine nucleotide exchange factor). The GEF displaces GDP from the binding site of RhoG and allows GTP to bind in it place rendering the protein active. This active RhoG binds downstream effector molecules that include ELMO and DOCK180. RhoG then cleaves GTP to GDP making it inactive. RhoG is believed to be crucial for integrin-mediated Rac activation and cell spreading. RhoG is believed to be active upstream of Rac and work independently of Rac.

Immunogen

KLH synthetic peptide encompassing amino acids CQQDGVKEVFAEAVRAVLNPT (RhoG 160-180)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monoclonal IgG2b in storage buffer containing PBS with 0.05% sodium azide.

Quality

Routinely evaluated by western blot on NIH/3T3 cell lysate.

Specificity

Recognizes RhoG

Target description

21 kDa