Analysis Note

ControlIncludes pervanadate treated HEK293 lysate as a positive control.

Application

Research CategorySignaling

Research Sub CategoryInsulin/Energy Signaling

Anti-phospho-Insulin Receptor Antibody, clone 21G12 is an antibody against phospho-Insulin Receptor (Tyr 1322) for use in ELISA & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyrosine 1322 on phosphorylated insulin receptor

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.

Format: Purified

Subsequent thiophilic adsorption and size exclusion chromatography

Quality

Routinely evaluated by immunoblot.

Specificity

Recognizes phosphorylated domain of Insulin receptor on Tyrosine 1322.

Storage and Stability

2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H₂O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

97 kDa