Application

Rabbit-Anti-Human IgG4 Antibody, clone RM120 is an antibody against Human IgG4 for use in Western Blotting, Immunohistochemistry, ELISA.

Immunohistochemistry Analysis (IHC): 1 -10 µg/mL from a representative lot detected human IgG4 in human lymphoid tissue.

ELISA Analysis: The specificity and reactivity of a representative lot was confirmed by ELISA analyses.

Research CategorySecondary & Control Antibodies

Research Sub CategorySecondary Antibodies Adsorbed for Dual Labeling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Peptide corresponding to the hinge region of Human IgG4.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in human, mouse, rat, and goat IgG.

Western Blotting Analysis (WB): 2 µg/mL of this antibody detected human IgG in human, mouse, rat, and goat IgG.

Specificity

This antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

(~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and
mouse, rat, or goat IgG.