Application

This Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.

Research CategorySecondary & Control Antibodies

Research Sub CategorySecondary Antibodies Adsorbed for Dual Labeling

Immunoprecipitation Analysis (IP): 0.5-2 µg/mL from a representative lot detected Histidine Tag in HEK293T whole lysate control and IP by clone RM146.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HEK293T cell lysate untransfected with His-Tag fusion protein X, transfected with His-Tag fusion protein X, E. coli lysate without His-Tag Protein Y, and E. coli lysate with His-Tag Protein Y.

Western Blotting Analysis (WB): 0.2 µg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.

Specificity

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~52 and ~85 kDa observed. Uncharacterized bands may be observed in some lysate(s).