Analysis Note

ControlHeLa cell lysate

Application

Anti-Centrin Antibody, clone 20H5 is a Mouse Monoclonal Antibody for detection of Centrin also known as Caltractin isoform 2, EF-hand protein & has been validated in WB, IP, ICC.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: Manandhar, G., et al. (1999). Cell Motil Cytoskeleton. 43(2):137-144.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Centrin is a key regulator of centriole duplication. This protein is localized to the centrosome of interphase cells. During mitosis, centrin redistributes to the spindle poles, consistent with dynamic changes of the centrosome during the cell cycle. Current research suggests centrin exerts spatial and temporal control over centriole duplication through its role as a component of centriole precursor structures and through periodic cell-cycle specific changes in its abundance.

Immunogen

Recombinant protein corresponding to the C-terminus of Chlamydomonas Centrin.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Centrin on 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes the C-terminus of Centrin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 20 kDa