Analysis Note

ControlHepG2 cell lysate

Application

Research Sub CategoryRNA Metabolism & Binding ProteinsTranscription Factors

Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected TLS in C2C12 cells.

Use Anti-TLS Antibody, clone 1FU-1D2 (Mouse Monoclonal Antibody) validated in WB, ICC to detect TLS also known as 75 kDa DNA-pairing protein, FUS-CHOP fusion protein.

Research CategoryEpigenetics & Nuclear FunctionEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The TLS/FUS protein contains an RNA-recognition motif and is a component of nuclear riboprotein complexes. It resembles a transcription factor in that it binds DNA, contributes a transcriptional activation domain to the FUS-ERG oncoprotein, and interacts with several transcription factors in vitro. It binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. It may play a role in maintenance of genomic integrity.

Immunogen

Linear peptide corresponding to human TLS.

Epitope: Unknown

Physical form

Unpurified mouse monoclonal IgG1κ preservative-free ascites.

Unpurified

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected TLS on 10 µg of HepG2 cell lysate.

Specificity

This antibody recognizes TLS.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Observed at 65 kDa