PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.
- Flexible protocols with the ability to use gravity flow or centrifugation
- Convenient desalting and buffer exchange of sample volumes of 0.1mL to 0.5mL for gravity and 0.2mL to 0.5mL with centrifugation
- Replaces dialysis for desalting protein, which saves time and reduces labour
- Filled with Sephadex G-25 Superfine resin
PD MiniTrap G-25 is designed for convenient desalting of biological samples. For increased flexibility, the protein desalting column can be used with either gravity or centrifugation.
When using the gravity protocol, clean-up of the sample is simple. One or several columns can be connected in parallel, and a purification system is not needed. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge. The eluted sample will have minimal dilution.
Four adapters are included in each product pack. The adapters simplify centrifugation of these protein desalting columns.
The exclusion limit is approximately Mr 5000. This ensures group separation of proteins/peptides larger than Mr 5000 from smaller molecules.
This product has met the following criteria to qualify for the following awards: