Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

HiTrap^® Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.The medium, Benzamidine Sepharose^® 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC^™ System.

Features and Benefits

Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates. HiTrap^® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. High binding capacity. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

General description

HiTrap^® Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose^® 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.

Legal Information

Sepharose is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

FPLC is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva