HiTrap rprotein A Fast Flow, Cytiva, 17-5079-01, pack of 5 x 1 mL

Code: GE17-5079-01 D2-231

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Quick, convenient preparat...


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€1,066.30 EACH
€1,311.55 inc. VAT
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Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Quick, convenient preparative purification of monoclonal antibodies. Well-established protein A-based medium used in many approved MAb processes. Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification. Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.

General description

HiTrap® rProtein A FF columns are prepacked with rProtein A Sepharose® Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose® Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

Legal Information

Sepharose is a registered trademark of Cytiva

HiTrap is a registered trademark of Cytiva

average diameter90 µm (d50v)
bed size7 mm × 25 mm
bed volume1 mL
capacity~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
cleaning2-11(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
column I.D.7 mm
manufacturer/tradenameCytiva 17-5079-01
matrix4% cross-linked agarose
packagingpack of 5 × 1 mL
parameter﹤4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi
particle size60-165 µm
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
storage condition(20% Ehtanol), (20% Ethanol)
storage temp.2-8°C
suitabilitysuitable for bioprocess medium
working range3-10
Hazard Class3
Un Number1170
Pack GroupIII
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