Application

Rapid ChemiAP Substrate is supplied as a ready-to-use aqueous solution, i.e., no dilution is necessary, for use in a variety of membrane-based applications such as Western Blotting and ELISA. Other applications include Southern, Northern Blotting, detection of placental alkaline phosphatase, detection of secreted alkaline phosphatase, reporter gene assays, nucleic acid probe assays and alkaline or acid phosphatase biomarker detection (stem cells and tumor markers).

Caution

Do not use diluents or wash buffers that contain EDTA or other chelators as they can remove divalent cations (Mg⁺², Zn⁺²) and cause loss of AP activity.

General description

Rapid ChemiAP Substrate is a sensitive, chemiluminescent substrate for alkaline phosphatase that allows for the rapid, reproducible detection of alkaline phosphatase-labeled molecules