General description

D-Gluconic acid (C6H12O7) is a mild organic acid that occurs from the oxidation of glucose. It is naturally present in fruit, honey, and wine When used as a food additive, it helps regulate acidity. It is a strong chelating agent, chelating the anions of calcium, iron, aluminum, copper, and other heavy metals. D-Gluconate (C6H11O7) is the salt or ester of Gluconic Acid. Due to its low toxicity, it is widely used in pharmaceutical, food, and other industries.

Principle

The D-Gluconate (gluconic acid) Assay Kit is a simple and sensitive method for quantifying D-gluconate. In this assay, gluconate is utilized by glucokinase to form D-gluconate-6-phosphate and ADP producing a coupled enzyme reaction that generates a colorimetric signal (450 nm), proportional to the amount of D-gluconate present. The assay is sensitive to less than 2 µM of D-gluconate in a variety of samples.

Suitability

Suitable for the measurement of D-Gluconate (D-Gluconic Acid) in various samples such as tissues, plants, fruits and wine