Application

Specialized culture media supplement, designed to isolate and cultivate various Mycobacterial species.

Components

Ingredients: (per vial)Bovine albumin fraction V 2.5gDextrose 1.0gCatalase 0.0015gDistilled water 50 ml

General description

Middlebrook ADC growth supplement is used to isolate a wide variety of Mycobacteria including M. tuberculosis. It contains Bovine albumin, dextrose, and catalase. Bovine albumin neutralizes the toxic products. Dextrose is the fermentable sugar and catalase cleaves the toxic hydrogen peroxides which creates stress to the Mycobacteria cells thus, making it an ideal media for the cultivation of Mycobacterial species

Reconstitution

Warm up the refrigerated supplement to 45-50°C. Aseptically add the contents of the vial to 450 ml of sterile Middlebrook 7H9 Broth Base (M0178). Mix well and pour into sterile screw capped tubes.