BTT Activator 0.3M OP

Code: L033000-4X2.5L N1-1

Other Notes

Activator solution, containing 5-(Benzylthio)-1H-Tetrazole (BTT or BMT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step. T...


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Other Notes

Activator solution, containing 5-(Benzylthio)-1H-Tetrazole (BTT or BMT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5'hydroxyl group of the growing oligonucleotide chain.

Preparation Note

Acetonitrile / 5-(Benzylthio)-1H-Tetrazole 100 / 6 (v/w)

InChI keyGXGKKIPUFAHZIZ-UHFFFAOYSA-N
Quality Level200
colorclear
storage conditiondry at room temperature
InChI1S/C8H8N4S/c1-2-4-7(5-3-1)6-13-8-9-11-12-10-8/h1-5H,6H2,(H,9,10,11,12)
product lineProligo Reagents
assay51.9-63.4 g/L (BTT content, titration)
typefor DNA synthesis
SMILES stringC(Sc1nnn[nH]1)c2ccccc2
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