Application

Immobilon-FL PVDF Membrane has been used in western blotting.

Features and Benefits

Immobilon-FL Membrane: The first transfer membrane optimized for fluorescence applications; Extremely low background enhances the sensitivity of all fluorescence detection protocols Compatible with all commonly used fluorescent probes at a wide range of excitation/emission wavelengths Best for multiplexing and chemifluorescence detections compatible with standard blocking agents and buffers

General description

The Immobilon^®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 µm. It is designed for binding proteins transferred from a variety of gel matrices. The Immobilon^®-FL transfer membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane offers optimal blotting for proteins with molecular weights greater than 20,000. The Immobilon^®-FL membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. Immobilon^®-FL membrane can also be used for standard chemiluminescent or chromogenic detection.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany