Application

Functional Genomics/Screening/Target Validation Non-targeting control, CRISPR lentivirus, viral format, for creating gene knockouts/genetic modifications in cell lines. To see more application data, protocols, vector maps visit sigma.com/crisprs.

CRISPR-Lenti Non-Targeting Control Transduction Particles has been used in the transduction of human embryonic kidney 293 luciferase expressing cells with lentiviral particles, isolation of single colonies and MOSPD2 (motile sperm domain containing protein 2) silencing.

Features and Benefits

Serves as a non-targeting or negative experimental control (lentiviral format) for the CRISPR editing workflow using lenti CRISPRs. This allows you to examine the effect of transduction in your system with the Sigma lenti CRISPR/Cas9 virus.

General description

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral system, which includes a gRNA sequence that does not target known human, mouse and rat genes. The non-targeting control lentivirus is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA (non-targeting), with both Puromycin and GFP co-expressed with Cas9. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).

Legal Information

CRISPR Use License AgreementLentiviral and WPRE License Agreements

Physical form

200 µl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.