CAV3.1 CALCIUM CHANNEL; RBX-200UL

Analysis Note

ControlIncluded free of charge with the antibody is 40 µg of control antigen (lyophilized powder). For negative control, preincubate 1 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Western blotting: 1:200 using ECL on rat brain membranes. See suggested protocol.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Research Sub CategoryIon Channels & TransportersNeuroinflammation & Pain

This Anti-Calcium Channel Cav3.1 Antibody is validated for use in WB for the detection of Calcium Channel Cav3.1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Highly purified peptide corresponding to residues 1-22 of rat Cav3.1 (Accession O54898).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.05% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Specificity

Recognizes Cav3.1. The epitope specific for Cav3.1 is not present in any other known proteins.

SPECIES REACTIVITIES:

The immunogen sequence is conserved in human (20/22). Reactivity with other species has not yet been tested.

Storage and Stability

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.