Application

Activation and coupling of particles > 1 µm in diameter

Features and Benefits

Water based, activation and coupling reagent for particles > 1 µm in diameter An alternative to traditional chemistries such as NHS/EDC methods with an easier to use AnteoBind protocol Reduce reagent preparation time by 3-4 hours Increase antibody functionality Low background noise High reproducibility between experiments

General description

AnteoBind Micro was developed for binding antibodies and other proteins to magnetic particles greater than 1 µm in size. It forms strong multi-valent interactions with almost any electron donating groups including carboxylic acids. Once proteins are added, AnteoBind activated particles rapidly form very strong but gentle binding interactions.AnteoBind Micro has been used to stably activate many particles. Examples include: M-270 Dynabeads^®, Bangs ProMag^®, JSR MS300 Carboxyl and Estapor^® M1-200/20.AnteoBind Micro has many advantages over existing methods such as EDC/NHS chemistry or alternative ″pre-activated″ methods such as Epoxy or Tosyl. AnteoBind is water based and replaces the dry chemicals you would use with the EDC method. Using AnteoBind instead of NHS/EDC chemistry can save up to 3-4 hours of lab time. Experiments have shown increased stability and functionality for antibodies and a decreased background. AnteoBind improves reproducibility between experiments by providing uniformity between surfaces. For particles from 0.5 µm to 1 µm the recommended AnteoBind product is AnteoBind Sub-Micron.

Legal Information

ProMag is a registered trademark of Polysciences, Inc

Estapor is a registered trademark of Merck KGaA, Darmstadt, Germany

Dynabeads is a registered trademark of Invitrogen Dynal AS Corp

Anteobind is a trademark of AnteoTech Ltd