TOYOPEARL Affinity Media, AF-Chelate-650M, 65 mum, bottle of 25 mL

Code: 814475 D2-231

Application

TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.

General...


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Application

TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.

General description

Toyopearl AF-Chelate-650 resin is derivatized with iminodiacetic acid for immobilized metal affinity chromatography or IMAC.IMAC works primarily by binding to histidine and free cysteine containing sequences of proteins or peptides.

Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Physical form

Shipped in 20% (v/v) ethanol.

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Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

descriptionAF-Chelate-650M
extent of labeling25-45 µeq/mL ligand density
formliquid
manufacturer/tradenameTosoh TOYOPEARL® AF, 14475
matrix active groupIDA phase, iminodiacetic acid
matrixhydroxylated methacrylic polymer
operating pH2-12
packagingbottle of 25 mL
parameter3 bar max. pressure
particle size65 µm
product lineTOYOPEARL®
separation techniqueaffinity
storage temp.2-8°C
technique(s)HPLC: suitable
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