Application
TOYOPEARL® media are used in hydrophobic interaction media, resins and separation media. TOYOPEARL® media offer high yield recovery of proteins, using various aqueous eluants.
General description
TOYOPEARL® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
Other Notes
Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps
Physical form
Shipped in 20% (v/v) ethanol.
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Specifications
Clean in place with 0.5 M NaOH or 0.1 M HCl.
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