Components

82 mlSpinPrep Bind Bufferr> 27 mlSpinPrep Wash Bufferr> 10 mlSpinPrep Elute Bufferr> 100SpinPrep Filtersr> 100Receiver Tubesr> 100Eluate Receiver Tubes

General description

Rapid purification of PCR products for downstream procedures

The SpinPrep﹤TMSYMBOL>﹤/TMSYMBOL> PCR Clean-Up Kit is designedfor the rapid purification of DNA amplifiedin PCR. The 10-minute procedure involvesaddition of a binding buffer followed byadsorption of the DNA to a silica membrane ina spin column format. Following a wash step,the DNA is eluted in low-salt buffer. This kitremoves DNA polymerases, dNTPs, salts, and> 99% of primers so that they do not interferewith downstream applications such as cloning,sequencing, or labeling. PCR products from100 bp to > 12,000 bp can be cleaned up, withrecoveries of amplified DNA in the range of60-90% under standard conditions.Column binding capacity: up to 6 gPCR volume: 100 l/rxnTypical recovery: 6090%Size range: 100 bp to > 12,000 bpTime required:﹤ 10 min

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100 reactions in Plastic ampoule

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)