General description
Streptavidin Agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination. Provided as a 50% slurry in storage buffer; 5 ml settled resin in 10 ml total volume.
Column or batch isolation of biotinylated molecules
| Specifications |
| Concentration | 50% suspension (5 ml settled volume) |
| Storage buffer | 100 mM sodium phosphate pH 7.5, 0.02% sodium azide |
| Size | 40-165 µm |
| Binding capacity | >85 nmol free biotin/ml |
| Protease | None detected (SDS-PAGE of purified protein) |
| DNase | None detected (blue/white colony assay of ligated plasmid) |
| RNase | None detected (gel assay of purified RNA) |
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging
5 ml in Glass bottle
Warning
Toxicity: Standard Handling (A)
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