General description
RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging
1000 u in Glass bottle
Unit Definition
One unit will degrade 1 µg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl2, 10 mM DTT, 1 µg plasmid DNA, and enzyme.
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria to qualify for the following awards: