Components

50 URecombinant Enterokinaser> 2 ml1X rEK Dilution/Storage Bufferr> 1 ml10X rEK Cleavage Bufferr> 10 µgCleavage Control Protein

General description

Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.

Packaging

50 u in Plastic ampoule

Unit Definition

One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)