Bolton Broth, Base, for microbiology

Code: 67454-500G D2-231

Application

for the selective enrichment of Campylobacter species from foods

Footnote

We offer two media types: the superior granulated GranuCult...


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€186.00 EACH
€228.78 inc. VAT
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Application

for the selective enrichment of Campylobacter species from foods

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Bolton broth base is used for the selective enrichment of Campylobacter species from foods as recommended by ISO Committee under specifications ISO 10272-1:2006 (E).

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

Suspend 13.8 grams in 472.5 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1vial of Bolton Selective Supplement (Cat. No. 40568) and 25 ml of sterile lysed defribinated horse blood. Mix well before dispensing into sterile tubes.

agencyaccording to ISO 10272-1:2017
application(s)microbiology, clinical testingfood and beveragespathogen testingsample preparation
compositionsodium chloride, 5.0 g/L, sodium metabisulfite, 0.5 g/L, α-ketoglutaric acid, 1.0 g/L, lactalbumin hydrolysates, 5.0 g/L, sodium carbonate, 0.6 g/L, yeast extract, 5.0 g/L, enzymatic digest of animal tissues, 10 g/L, sodium pyruvate, 0.5 g/L
final pH7.4±0.2 (25 °C)
formpowder
manufacturer/tradenameNutriSelect® Plus
Quality Level200
shelf lifelimited shelf life, expiry date on the label
sterilitynon-sterile
suitabilityselective for Campylobacter spp.
technique(s)microbiological culture: suitable
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