Application

Pronase^® Protease, Streptomyces griseus, nuclease-free has been used:in single-cell preparations for cardiac side population (CSP) analysisin aflatoxin plasma biomarker assayto permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion

Biochem/physiol Actions

Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.

General description

PRONASE^® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.

Legal Information

PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Note: 1 KU = 1000 units.

Packaging

25 ku in Glass bottle

10, 100, 5, 50 ku in Plastic ampoule

Reconstitution

Unstable in solution; reconstitute just prior to use.

Unit Definition

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Warning

Toxicity: Harmful (C)