General description

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.

Note: 1 KU = 1000 units.

Legal Information

PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Narahashi, Y., et al. 1968. J. Biochem. 64, 427.

Packaging

10, 25, 250, 50, 500 ku in Plastic ampoule

Physical form

Lyophilized from a solution containing calcium acetate as a stabilizer.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.

Unit Definition

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Warning

Toxicity: Harmful (C)