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HEPES buffered saline, BioUltra, for molecular biology, 2x concentrate

Code: 51558-50ML D2-231

Application

HEPES buffered saline has been used:for the preparation of plasmid DNA as a reagent to prepare transfection master mix for DNA construct vectorsto develop hydroge...


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€78.70 50ML
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Application

HEPES buffered saline has been used:for the preparation of plasmid DNA as a reagent to prepare transfection master mix for DNA construct vectorsto develop hydrogel precursor for cell leakage assay

Components

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

λneat
cation tracesCd: ≤1 mg/kg, Fe: ≤1 mg/kg, Mg: ≤1 mg/kg, Bi: ≤1 mg/kg, Mo: ≤1 mg/kg, Al: ≤1 mg/kg, Ni: ≤1 mg/kg, Cr: ≤1 mg/kg, Ca: ≤5 mg/kg, Zn: ≤1 mg/kg, Pb: ≤1 mg/kg, Co: ≤1 mg/kg, Ba: ≤1 mg/kg, Cu: ≤1 mg/kg, Mn: ≤1 mg/kg, Sr: ≤1 mg/kg, Li: ≤1 mg/kg
compositionNa2HPO4.2H2O, 0.27 g/L, HEPES, 10 g/L, NaCl, 16 g/L, dextrose, 2.0 g/L, KCl, 0.74 g/L
concentration2 ×
formliquid
gradefor molecular biology
impuritiesphosphatases, none detected, RNases, none detected, proteases, none detected, DNases, none detected
InChI keyJKMHFZQWWAIEOD-UHFFFAOYSA-N
InChI1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
pH7.2 (25 °C)
product lineBioUltra
Quality Level200
quality2× concentrate
refractive indexn20/D 1.339
SMILES stringOCCN1CCN(CC1)CCS(O)(=O)=O
UV absorptionλ: 280 nm Amax: 0.04, λ: 260 nm Amax: 0.06
This product has met the following criteria: