Biochem/physiol Actions
Primary Targetceramide synthase
Target IC50: 100 nM against sphingosine N-acyltransferase (ceramide synthase)
General description
A cell-permeable mycotoxin that inhibits sphingolipid biosynthesis in rat kidney and in liver microsomes by inhibition of sphingosine N-acyltransferase (ceramide synthase; IC50 = 100 nM). Preferentially inhibits sphingomyelin biosynthesis in neuronal cells. Exhibits carcinogenic properties.
Fumonisin B1 is a neurotoxin and a phytotoxin. A cell-permeable mycotoxin that inhibits sphingolipid biosynthesis in rat kidney and in liver microsomes by inhibition of sphingosine N-acyltransferase (ceramide synthase) (IC50 = 100 nM). Cellular effects also appear to be induced by micromolar levels of FB1. Because it inhibits ceramide synthase activity, it elevates cellular levels of sphingoid bases, including sphinganine, resulting in overall inhibition of sphingolipid biosynthesis. Preferentially inhibits sphingomyelin biosynthesis in neuronal cells. Has carcinogenic properties.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Wu, W.I., et al. 1995. J. Biol. Chem.270, 13171.Riley, R.T., et al. 1994. J. Nutr. 124, 594.Wolf, G. 1994. Nutr. Rev. 52, 246.Merrill, A.H. Jr., et al. 1993. Adv. Lipid Res.26, 215.Merrill, Jr., A.H., et al. 1993. J. Biol. Chem.268, 27299.Voss, K.A., et al. 1993. Nat. Toxins1, 222.Wang, E., et al. 1991. J. Biol. Chem.266, 14486.Harrison, L.R., et al. 1990. J. Vet. Diag. Invest. 2, 217.
Packaging
1 mg in Plastic ampoule
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Warning
Toxicity: Carcinogenic / Teratogenic (D)
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