Application

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS’; small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

General description

Zwitterionic detergent ideal for solubilization of membrane proteins, analytical, diagnostic, chromatography, electrophoresis work, and enzymology and immunology applications.Suitable for Molecular BiologyConductivity (10%, water): ≤50 µmhoDNase: None detectedProtease: None detectedPurity (TLC): Single spotRNase: None detectedSolubility: 20% in water, passes test Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Legal Information

OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100 g in Plastic bottle