DAPI; Dihydrochloride

Biochem/physiol Actions

Cell permeable: no

Primary TargetBinds preferentially to DNA rich in adenine and thymine

Reversible: yes

Product does not compete with ATP.

General description

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H₂O (Cat. No. 508741).

Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.Hotz, M.A., et al. 1994. Cytometry15, 237.Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.Miller, T., et al. 1993. Biotechniques15, 1042.Gray, J.W., et al. 1982. Science238, 323.Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.Russels, W.C., et al. 1975. Nature253, 461.

Packaging

10 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)