1;5-Dansyl-Glu-Gly-Arg Chloromethyl Ketone; Dihydrochloride

Biochem/physiol Actions

Cell permeable: no

Primary TargetUPA

Reversible: no

Product does not compete with ATP.

Target IC₅₀: 100 nM for factor Xa

General description

Effective irreversible inhibitor of uPA, Factor Xa, and urokinase. Fluorophore allows direct monitoring of interactions of labeled enzyme. Does not inhibit tPA.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bajaj, S.P., et al. 1992. Proc. Natl. Acad. Sci. USA89, 152.Bergmeyer, H.V. 1984. Methods Enzym. Anal. 5, 375.Kettner, C., and Shaw, E. 1981. Methods Enzymol.80, 826.

Packaging

5 mg in Plastic ampoule

Reconstitution

Following initial thaw, aliquot and refrigerate (4°C). Stock solutions are stable for up to 4 months at 4°C.

Reconstitute at 1 mg/ml in 10 mM HCl. Dilute with physiological buffers just prior to use.

Warning

Toxicity: Standard Handling (A)