Application
5(6)-Carboxyfluorescein diacetate (CFDA) is a dye which is been extensively utilized to label cultured neurons with the essential requirements, in a specific manner. 5(6)- Carboxyfluorescein diacetate (CFDA) is a viability probe that assesses enzymatic activity, which is necessary to activate its fluorescence, and cell-membrane integrity, which is necessary for the retention of their fluorescent product within the cell.
General description
5(6)-Carboxyfluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.
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