Caspase-8 Inhibitor II

Analysis Note

single spot by TLC

Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Primary Targetcaspase-8

Cell permeable: yes

General description

A potent, cell-permeable, and irreversible inhibitor of caspase-8 (MACH, FLICE, Mch5). Also inhibits granzyme B.

A potent, cell-permeable, and irreversible inhibitor of caspase-8 and granzyme B. Effectively inhibits influenza virus-induced apoptosis in HeLa cells. Also inhibits granzyme B. When using with purified native or recombinant enzyme, pretreatment with an esterase is required. A 5 mM (250 µg/76 µl) solution of Z-IETD-FMK (Cat. No. 218840) in DMSO is also available.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Takizawa, T., et al. 1999. Microbiol. Immunol.43, 245.Martin, D.A., et al. 1998. J. Biol. Chem.273, 4345.Sweeney, E.A., et al. 1998. FEBS Lett.425, 61.Thornberry, N.A., and Lazebnik, Y. 1998. Science281, 1312.

Packaging

1 mg in Plastic ampoule

250 µg in Glass bottle

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 8 months at -20°C.

Sequence

Z-Ile-Glu(OMe)-Thr-Asp(OMe)-CH₂F*

Warning

Toxicity: Standard Handling (A)